Journal: Cell

Article Title: The 7q11.23 Protein DNAJC30 is an Auxiliary Component of ATP Synthase and Links Mitochondria to Brain Development

doi: 10.1016/j.cell.2018.09.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Avidin-Biotin blocker , Vector Laboratories , Cat#SP-2001.

Techniques: Recombinant, Protease Inhibitor, Modification, Avidin-Biotin Assay, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Knock-Out, Sequencing, Clone Assay, Software, Microscopy, Digital PCR, Fluorescence, Transmission Assay

Journal: Science Advances

Article Title: Interference with pancreatic sympathetic signaling halts the onset of diabetes in mice

doi: 10.1126/sciadv.abb2878

Figure Lengend Snippet: ( A ) Depletion of macrophages from RIP-LCMV-GP mice by clodronate liposomes during onset of T1D was protective against the development of hyperglycemia (Student’s t test on day 12, * P < 0.05 on day 12). ( B to E ) Pancreatic macrophages in healthy, uninfected mice expressed both α 1 and β 2 adrenoceptors, and intra-islet macrophages had a higher expression of β 2 adrenoceptors (C) and tendencies of higher expression of α 1 adrenoceptors (B) than exocrine macrophages (flow plots representative of n = 4 mice). (D) A biotinylated nonselective β blocker, alprenolol, was used as an additional detection method for β adrenoceptors. Mean fluorescence intensities of receptor staining from four mice (Student’s t test, * P < 0.05) are presented in (E). FMO, fluorescence minus one. a.u., arbitrary units. ( F ) Isolated islet macrophages were stimulated with 10 −6 and 10 −8 M norepinephrine (NE) in vitro and shifted their expression of cytokines. The heatmap displays log 2 transforms of quantitative cytokine concentrations in cell lysates (absolute values in fig. S3). ( G ) Islet-resident macrophages were isolated and stimulated with 10 −8 M NE in the presence or absence of an α 1 (prazosin) or a β 2 (butoxamine) adrenoceptor antagonist ( n = 3 mice in two independent experiments, one-way ANOVA). # P < 0.05 compared to unstimulated control and ¤ P < 0.05 compared to the NE-stimulated group.

Article Snippet: A biotinylated nonselective β blocker (biotin-alprenolol; CellMosaic) was used as an alternative approach to detect β adrenoceptors. eBioscience strepatavidin phycoerythrin (Thermo Fisher Scientific) was used for fluorescence detection.

Techniques: Liposomes, Expressing, Fluorescence, Staining, Isolation, In Vitro, Control

Journal: Sensors (Basel, Switzerland)

Article Title: A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry

doi: 10.3390/s20205745

Figure Lengend Snippet: Growth of the biotin-streptavidin lawn. The overnight (O/N) incubation conditions were H 2 O, PBS, or a mixture of amine-PEG 11 -biotin and amine-PEG 4 -methyl. Thicknesses are the averages of three chips measured via ellipsometry and fit to a model of silicon dioxide layer on silicon substrate after each step of the growth process. Error bars represent the standard deviation of thickness measurements between three replicate chips. All chips were washed with Nanopure TM water and dried with a nitrogen gas stream after each incubation and prior to ellipsometry except after streptavidin where one set of chips (indicated above) was allowed to dry in ambient air on the laboratory benchtop. Streptavidin only builds onto the chips exposed to amine-biotin, and a significant loss of surface thickness is observed after drying and rewetting with assay wash buffer (AWB) after either drying condition.

Article Snippet: Streptavidin, avidin, anti-fluorescein (FITC) (goat polyclonal), bovine serum albumin (BSA), and human IgG were purchased from Rockland Immunochemicals.

Techniques: Incubation, Standard Deviation